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Membrane Protein Structure and Function Unit - Division of Intramural Research



2013

Grisshammer R
Why we need many more GPCR structures, Expert Review of Proteomics, 2013, vol. 10, pp. 1-3.

Shibata Y, Gvozdenovic-Jeremic J, Love J, Kloss B, White JF, Grisshammer R, Tate CG
Optimising the combination of thermostabilising mutations in the neurotensin receptor for structure determination, Biochim Biophys Acta Biomembranes, 2013, vol. 1828, pp. 1293-1301.

Xiao S, White JF, Betenbaugh MJ, Grisshammer R, Shiloach J
Transient and stable expression of the neurotensin receptor NTS1: A comparison of the baculovirus-insect cell and the T-REx-293 expression systems, PlosOne, 2013, vol. 8, pp. e63679.

Niesen MJM, Bhattacharya S, Grisshammer R, Tate CG, Vaidehi N
Thermostabilization of the β1-adrenergic receptor correlates with increased entropy of the inactive state, J Phys Chem B, 2013, vol. 117, pp. 7283-7291.

Inagaki S, Ghirlando R, Grisshammer R
Biophysical characterization of membrane proteins in nanodiscs, Methods, 2013, vol. 59, pp. 287-300. Full Text/Abstract

2012

Inagaki S, Ghirlando R, White JF, Gvozdenovic-Jeremic J, Northup JK, Grisshammer R
Modulation of the interaction between neurotensin receptor NTS1 and Gq protein by lipid, J Mol Biol, 2012, vol. 417, pp. 95-111.

White JF, Noinaj N, Shibata Y, Love J, Kloss B, Xu F, Gvozdenovic-Jeremic J, Shah P, Shiloach J, Tate CG, Grisshammer R
Structure of the agonist-bound neurotensin receptor, Nature, 2012, vol. 490, pp. 508-513.

2010

White JF, Grisshammer R
Stability of the neurotensin receptor NTS1 free in detergent solution and immobilized to affinity resin, PLoS One, 2010, vol. 5, pp. e12579.

2009

Grisshammer R
Purification of recombinant G-protein-coupled receptors, Methods in Enzymology, Guide to Protein Purification, Second Edition, 2009, vol. 463, pp. 631-645.

Shibata Y, White JF, Serrano-Vega MJ, Magnani F, Aloia AL, Grisshammer R, Tate CG
Thermostabilization of the neurotensin receptor NTS1, J Mol Biol, 2009, vol. 390, pp. 262-277.

2008

Ho JTC, White JF, Grisshammer R, Hess S
Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography-electrospray ionization-mass spectrometry, Analytical Biochemistry, 2008, vol. 376, pp. 13-24.

2007

White JF, Grodnitzky J, Louis JM, Trinh LB, Shiloach J, Gutierrez J, Northup JK, Grisshammer R
Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction, Proc. Natl. Acad. Sci. USA, 2007, vol. 104, pp. 12199-12204.

White JF, Grisshammer R
Automated large-scale purification of a recombinant G-protein-coupled neurotensin receptor, Current Protocols in Protein Science, 2007, vol. unit 6.8, pp. 6.8.1-6.8.31.

2006

Grisshammer R, Buchanan SK (editors)
Structural Biology of Membrane Proteins, Royal Society of Chemistry book, 2006, vol. ISBN 0 85404 361 6

Grisshammer R
Review 'Understanding recombinant expression of membrane proteins', Current Opinion in Biotechnology, 2006, vol. 7, pp. 337-340.

Niebauer RT, White JF, Fei Z, Grisshammer R
Characterization of monoclonal antibodies directed against the rat neurotensin receptor NTS1, Journal of Receptors and Signal Transduction, 2006, vol. 26, pp. 395-415.

2005

Grisshammer R, White JF, Trinh LB, Shiloach J
Large-scale expression and purification of a G-protein-coupled receptor for structure determination - an overview, Journal of Structural and Functional Genomics, 2005, vol. 6, pp. 159-163.

2004

White JF, Trinh LB, Shiloach J, Grisshammer R
Automated large-scale purification of a G-protein-coupled receptor for neurotensin, FEBS Letters, 2004, vol. 564, pp. 289-293.

2003

Luca S, White JF, Sohal AK, Filippov DV, v. Boom JH, Grisshammer R, Baldus M
The conformation of neurotensin bound to its G-protein-coupled receptor, Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 10706-10711.

Grisshammer R, Tate CG (editors)
Special Issue on Overexpression of Integral Membrane Proteins, Biochimica et Biophysica Acta (BBA), 2003, vol. 1610, pp. 1-153.

2002

Weiss HM, Grisshammer R
Purification and characterisation of the human adenosine A2a receptor functionally expressed in Escherichia coli, Eur J Biochem, 2002, vol. 269, pp. 82-92.

Daniels DA, Sohal AK, Rees S, Grisshammer R
Generation of RNA aptamers to the G-protein-coupled receptor for neurotensin NTS1, Anal Biochem, 2002, vol. 305, pp. 214-226. Full Text/Abstract

Grisshammer R, Grunwald T, Sohal AK
Characterization of an antibody Fv fragment that binds to the human but not to the rat neurotensin receptor NTS1, Protein Expr Purif, 2002, vol. 24, pp. 505-512. Full Text/Abstract

2001

Kunji ER, Spudich EN, Grisshammer R, Henderson R, Spudich JL
Electron crystallographic analysis of two-dimensional crystals of sensory rhodopsin II: A 6.9 A projection structure, J Mol Biol, 2001, vol. 308, pp. 279-293. Full Text/Abstract

Grisshammer R, Hermans E
Functional coupling with Galpha(q) and Galpha(i1) protein subunits promotes high-affinity agonist binding to the neurotensin receptor NTS-1 expressed in Escherichia coli, FEBS Letters, 2001, vol. 493, pp. 101-105. Full Text/Abstract

1999

Grisshammer R, Averbeck P, Sohal AK
Improved purification of a rat neurotensin receptor expressed in Escherichia coli, Biochem Soc Trans, 1999, vol. 27, pp. 899-903. Full Text/Abstract

Grisshammer R, Tucker J
Expression in Escherichia coli and large-scale purification of a rat neurotensin receptor, Book chapter 10 in 'G Protein-Coupled Receptors' Methods in Signal Transduction Series, 1999, pp. 265-280.

1998

Grisshammer R
Expression of G protein-coupled receptors in Escherichia coli. Book chapter 7 in 'Identification and Expression of G Protein-Coupled Receptors' (volume editor: Kevin Lynch), Receptor Biochemistry and Methodology Series, 1998, pp. 133-150.

1997

Grisshammer R, Tucker J
Quantitative evaluation of neurotensin receptor purification by immobilized metal affinity chromatography, Protein Expr Purif, 1997, vol. 11, pp. 53-60. Full Text/Abstract

Calandra B, Tucker J, Shire D, Grisshammer R
Expression in Escherichia coli and characterization of the human central CB1 and peripheral CB2 cannabinoid receptors, Biotechnology Letters, 1997, vol. 19, pp. 425-428.

1996

Tate CG, Grisshammer R
Heterologous expression of G-protein-coupled receptors, Trends Biotechnol, 1996, vol. 14, pp. 426-430. Full Text/Abstract

Tucker J, Grisshammer R
Purification of a rat neurotensin receptor expressed in Escherichia coli, Biochem J, 1996, vol. 317, pp. 891-899. Full Text/Abstract

Popp MP, Grisshammer R, Hargrave PA, Smith WC
Ant opsins: Sequences from the Saharan silver ant and the carpenter ant, Invertebrate Neuroscience, 1996, vol. 1, pp. 323-329.

1995

Grisshammer R, Tate CG
Overexpression of integral membrane proteins for structural studies, Q Rev Biophys, 1995, vol. 28, pp. 315-422. Full Text/Abstract

1994

Grisshammer R, Little J, Aharony D
Expression of rat NK-2 (neurokinin A) receptor in E. coli, Receptors and Channels, 1994, vol. 2, pp. 295-302.

1993

Grisshammer R, Duckworth R, Henderson R
Expression of a rat neurotensin receptor in Escherichia coli, Biochem J, 1993, vol. 295, pp. 571-576. Full Text/Abstract

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