The purpose of this Funding Opportunity Announcement (FOA), sponsored by multiple NIH Institutes/Centers (ICs), is to promote the use of metabolomic technologies in translational research in human health and disease for the purposes of enabling and improving disease detection, diagnosis, risk assessment, prognosis, and prediction of therapeutic responses. In addition to this overall goal, specific scopes and objectives of the participating ICs are presented below. Using the NIH Research Project Grant (R01) funding mechanism, this FOA focuses on well developed projects supported by preliminary data.